Molecular Biology

A Project Approach

This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented.

Molecular Biology is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning.

• Includes a glossary of over 200 terms important for understanding molecular biology

• Uses an inexpensive source of eukaryotic cells - great for schools on a budget

• Includes Methods Locator that provides instant access to the latest methods

• Contain clearly written, easy-to-follow, student-tested instructions:

• Sterile techniques

  Phage titration

  Gel electrophoresis of DNA

  Restriction enzyme digestion

  Plasmid isolation

  Transformation of E. Coli

  Recombinant DNA cloning

  Nick translation labeling

  Nonradioactive primer labelling

  Nonradioactive DNA detection

  Southern blotting

  Colony hybridization

  Purification of plant DNA

  RNA purification

  Northern blotting

  Purification of poly A+ RNA

  Polymerase chain reaction (PCR)